All lanes : Anti-DR5 antibody [EPR1659(2)] (ab181846) at 1/50000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : HT1080 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling DR5 with ab181846 at 1/400 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot analysis of HeLa cell lysate immunoprecipitated using ab181846 at 1/30 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG was used at 1/1500 dilution.