Anti-Drosha antibody

• Supplier: Abcam
• Catalog: ab58589
• Prices: $378.00
• Sizes: 200 µl
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P WB
• Species Reactivities: Rat, Human, Mouse, Dog
• Antigen: Synthetic peptide: C-KQTDKQKLAQRE , corresponding to internal sequence amino acids 811-822 of Human Drosha Run BLAST with Run BLAST with
• Description: Goat Polyclonal
• Gene: DROSHA
• Conjugate: Unconjugated

Product images

Anti-Drosha antibody (ab58589) at 0.5 µg/ml (incubated for 1 hour) + Human Liver lysate (35µg protein in RIPA buffer). developed using the ECL technique

IHC image of ab58589 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58589, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Role of miR-21 in the pathogenesis of atrial fibrosis. - Role of miR-21 in the pathogenesis of atrial fibrosis.

Adam O, Lohfelm B, Thum T, Gupta SK, Puhl SL, Schafers HJ, Bohm M, Laufs U. Basic Res Cardiol. 2012 Sep;107(5):278.

NMDA mediated contextual conditioning changes miRNA expression. - NMDA mediated contextual conditioning changes miRNA expression.

Kye MJ, Neveu P, Lee YS, Zhou M, Steen JA, Sahin M, Kosik KS, Silva AJ. PLoS One. 2011;6(9):e24682.