DROSHA

Gene Symbol	DROSHA
Entrez Gene	29102
Alt Symbol	ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN
Species	Human
Gene Type	protein-coding
Description	drosha, ribonuclease type III
Other Description	RNase III\|drosha, double-stranded RNA-specific endoribonuclease\|nuclear RNase III Drosha\|p241\|protein Drosha\|putative protein p241 which interacts with transcription factor Sp1\|putative ribonuclease III\|ribonuclease 3\|ribonuclease III, nuclear\|ribonuclease type III, nuclear
Swissprots	Q9Y4Y0 Q7Z5V2 Q86YH0 Q9Y2V9 E7EMP9 Q9NRR4 Q9NW73
Accessions	CBM42910 CBV35643 EAX10767 EAX10768 EAX10769 EAX10770 Q9NRR4 AA917625 AB209501 BAD92738 AF116910 AAD29637 AF189011 AAF80558 AJ242976 CAB45133 AK001121 BAA91511 AK026563 AK308093 BC024261 BC041162 AAH41162 BC054003 AAH54003 BX647724 DB065470 XM_005248291 XP_005248348 XM_005248292 XP_005248349 XM_005248293 XP_005248350 XM_005248294 XP_005248351 XM_011514033 XP_011512335 NM_001100412 NP_001093882 NM_013235 NP_037367
Function	Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA- ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double- strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. {ECO:0000269\|PubMed:10948199, ECO:0000269\|PubMed:14508493, ECO:0000269\|PubMed:15531877, ECO:0000269\|PubMed:15565168, ECO:0000269\|PubMed:15574589, ECO:0000269\|PubMed:15589161, ECO:0000269\|PubMed:16751099, ECO:0000269\|PubMed:16906129, ECO:0000269\|PubMed:17159994}.
Subcellular Location	Nucleus. Nucleus, nucleolus. Note=A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies.
Tissue Specificity	Ubiquitous. {ECO:0000269\|PubMed:10948199}.
Top Pathways	Ribosome biogenesis in eukaryotes, Proteoglycans in cancer

Drosha (D28B1) Rabbit mAb - 3364 from Cell Signaling Technology

Prices	$99.00, $246.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D28B1

Western blot analysis of extracts from various cell types using Drosha (D28B1) Rabbit mAb.

Drosha (D30F3) Rabbit mAb - 3410 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D30F3

Western blot analysis of extracts from various cell types using Drosha (D30F3) Rabbit mAb.

RNase III Drosha (E-19) - sc-31159 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

RNase III Drosha (E-19): sc-31159. Western blot analysis of RNase III Drosha expression in mouse brain (A) and human skeletal muscle (B) tissue extracts.

RNase III Drosha (C-7) - sc-393591 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

RNase III Drosha (C-7): sc-393591. Western blot analysis of RNase III Drosha expression in SW480 (A) and HeLa (B) nuclear extracts.

RNase III Drosha (H-300) - sc-33778 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

RNase III Drosha (I-16) - sc-31158 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-Drosha antibody - ab58589 from Abcam

Prices	$378.00
Sizes	200 µl
Host	Goat
Clonality	Polyclonal

Anti-Drosha antibody (ab58589) at 0.5 µg/ml (incubated for 1 hour) + Human Liver lysate (35µg protein in RIPA buffer). developed using the ECL technique

Anti-Drosha antibody - ab85027 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

ab85027 at 1/250 dilution staining Drosha in human ovarian tumor by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

Anti-Drosha antibody - ab135956 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Drosha antibody (ab135956) at 1/500 dilution + Mouse whole brain tissue extract at 30 µg

Anti-Drosha antibody [EPR12794] - ab183732 from Abcam

Prices	$384.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR12794

Immunofluorescent analysis of acetone-fixed HeLa cells labeling Drosha with ab183732 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 diution. Counter stained with Dapi (blue).

Anti-Drosha antibody - ChIP Grade - ab12286 from Abcam

Prices	$403.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Human female amniocytes immunostained with ab12286 Drosha (FITC) (1/25 dilution). The DNA is labelled red with propidium iodide. This image was submitted as part of a review by Ahmad Khalil.

Anti-Drosha antibody - N-terminal - ab199911 from Abcam

Host	Rabbit
Clonality	Polyclonal

Anti-Drosha antibody - N-terminal (ab199911) at 1/1000 dilution + mouse thymus total cell extracts

Anti-DROSHA / RNASEN Antibody (aa811-822) - LS-C55399 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Antibody (0.5 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-DROSHA / RNASEN Antibody (aa1114-1235) - LS-C296275 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-DROSHA / RNASEN Antibody (aa1050-1100) - LS-C286621 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Mouse Drosha by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Drosha used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.

Anti-DROSHA / RNASEN Antibody - LS-C314094 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-DROSHA / RNASEN Antibody (aa737-764, APC) - LS-C268357 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DROSHA / RNASEN Antibody (aa737-764, AP) - LS-C268356 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DROSHA / RNASEN Antibody (aa737-764, Biotin) - LS-C268358 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DROSHA / RNASEN Antibody (aa737-764, FITC) - LS-C268359 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DROSHA / RNASEN Antibody (aa737-764, PE) - LS-C268360 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DROSHA / RNASEN Antibody (aa737-764, Azide-free, HRP) - LS-C268361 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DROSHA / RNASEN Antibody (aa1324-1374) - LS-C288663 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-DROSHA / RNASEN Antibody (aa774-823) - LS-C291732 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-DROSHA / RNASEN Antibody (C-Terminus) - LS-C110561 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

DROSHA / RNASEN antibody LS-C110561 Western blot of Fetal Spleen lysate.

Search more