Immunofluorescent analysis of acetone-fixed HeLa cells labeling Drosha with ab183732 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 diution. Counter stained with Dapi (blue).
Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling Drosha with ab183732 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
All lanes : Anti-Drosha antibody [EPR12794] (ab183732) at 1/50000 dilutionLane 1 : Jurkat cell lysateLane 2 : 293 cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution