Anti-Drosha antibody - ChIP Grade
| Name | Anti-Drosha antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab12286 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IP WB ICC/IF IHC-P ChIP |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide derived from within residues 1 - 100 of Human Drosha |
| Description | Rabbit Polyclonal |
| Gene | DROSHA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Human female amniocytes immunostained with ab12286 Drosha (FITC) (1/25 dilution). The DNA is labelled red with propidium iodide. This image was submitted as part of a review by Ahmad Khalil.
ab12286 at a 1/25 dilution staining human HeLa cells by immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a Cy3 conjugated donkey anti-rabbit polyclonal antibody. This image is courtesy of an Abreview submitted on 7 February 2006.See Abreview
All lanes : Anti-Drosha antibody - ChIP Grade (ab12286) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/15000 dilutionPerformed under reducing conditions.
All lanes : Anti-Drosha antibody - ChIP Grade (ab12286) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Nuclear Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab12286 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12286, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ICC/IF image of ab12286 stained Hela cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12886, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
MicroRNA editing facilitates immune elimination of HCMV infected cells. - MicroRNA editing facilitates immune elimination of HCMV infected cells.
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Drosha as an interferon-independent antiviral factor. - Drosha as an interferon-independent antiviral factor.
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RNA-Seq of the nucleolus reveals abundant SNORD44-derived small RNAs. - RNA-Seq of the nucleolus reveals abundant SNORD44-derived small RNAs.
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Rbfox3 controls the biogenesis of a subset of microRNAs. - Rbfox3 controls the biogenesis of a subset of microRNAs.
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Clinical and functional impact of TARBP2 over-expression in adrenocortical - Clinical and functional impact of TARBP2 over-expression in adrenocortical
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Drosha regulates gene expression independently of RNA cleavage function. - Drosha regulates gene expression independently of RNA cleavage function.
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Expression of the RNase III enzyme DROSHA is reduced during progression of human - Expression of the RNase III enzyme DROSHA is reduced during progression of human
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T cell activation induces proteasomal degradation of Argonaute and rapid - T cell activation induces proteasomal degradation of Argonaute and rapid
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A competitive regulatory mechanism discriminates between juxtaposed splice sites - A competitive regulatory mechanism discriminates between juxtaposed splice sites
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Role of microRNAs and microRNA machinery in the pathogenesis of diffuse large - Role of microRNAs and microRNA machinery in the pathogenesis of diffuse large
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