![Overlay histogram showing HEK293 cells stained with ab140494 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140494, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/10315_ab140494-8-ab140494FC.jpg)
Overlay histogram showing HEK293 cells stained with ab140494 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140494, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling DNM1L with ab140494 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human kidney carcinoma tissue labelling DNM1L with ab140494 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling DNM1L with ab140494 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human ovary adenocarcinoma tissue labelling DNM1L with ab140494 at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling DNM1L with ab140494 at 1/150 dilution.
![All lanes : Anti-DRP1 antibody [2E7] (ab140494) at 1/200 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY DNM1L cDNALysates/proteins at 5 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/10321_DNM1L-Primary-antibodies-ab140494-6.jpg)
All lanes : Anti-DRP1 antibody [2E7] (ab140494) at 1/200 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY DNM1L cDNALysates/proteins at 5 µg per lane.
![All lanes : Anti-DRP1 antibody [2E7] (ab140494) at 1/200 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : SVT2 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/10322_DNM1L-Primary-antibodies-ab140494-7.jpg)
All lanes : Anti-DRP1 antibody [2E7] (ab140494) at 1/200 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : SVT2 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.