Frataxin Protein Quantity Dipstick Assay Kit

• Supplier: Abcam
• Catalog: ab109881
• Prices: $354.00, $627.00
• Sizes: 30 tests, 90 tests
• Assay Type: Sandwich (quantitative)
• Sample Type: Whole Blood
• Format: Reagents
• Applications: ELISA
• Species Reactivities: Human
• Gene: FXN

Product images

An example using ab109881 to quantify frataxin levels from β -lymphocyte cell culture samples derived from control individuals and Friedreich's Ataxia (FA) patients. Shown is a 1:2 dilution series using a positive control sample. Approximately 6 to 8 dipsticks are suitable for covering the entire working range and the blank for background levels. In this example the dilution series starts with 4 µg of control sample. A one-site hyperbola line was generated for best-fit analysis using GraphPad.

An example using ab109881 to quantify frataxin levels from β -lymphocyte cell culture samples derived from control individuals and Friedreich's Ataxia (FA) patients. Based on the above standard curve values, of protein extract for the controls and FA patient samples. (Note: for a statistical analysis, it is preferred to use two dipsticks for each sample; intra-assay CV's are typically <=10%.) For this analysis, the FA patients had between 550 - 925 GAA repeats on the smaller allele. Using GraphPad software, the signal intensity from the standard curve was interpolated and the relative amount of frataxin in the patients, as compared to the controls, was determined. Based on the above analysis, the patient samples had between 13% and 20% of frataxin levels compared to the control.

Dipstick assays use the well-established lateral flow concept, whereby capture antibodies are striped onto nitrocellulose membrane and a Whatman paper wicking pad draws the sample through the antibody bands. Detector antibodies, conjugated to gold, are dried in the wells of a 96-well plate. Sample is added to the well, the dipstick inserted, and within minutes the line for each target is revealed as the protein-detector antibody-gold complex binds with the capture antibodies. Multiplexing dipstick assays have multiple target protein lines. A positive control goat anti-mouse antibody line is included on all assays to ensure that adequate wicking of the sample occurred.

Product References

Usefulness of frataxin immunoassays for the diagnosis of Friedreich ataxia. - Usefulness of frataxin immunoassays for the diagnosis of Friedreich ataxia.

Deutsch EC, Oglesbee D, Greeley NR, Lynch DR. J Neurol Neurosurg Psychiatry. 2014 Sep;85(9):994-1002. doi:

Generation and characterisation of Friedreich ataxia YG8R mouse fibroblast and - Generation and characterisation of Friedreich ataxia YG8R mouse fibroblast and

Sandi C, Sandi M, Jassal H, Ezzatizadeh V, Anjomani-Virmouni S, Al-Mahdawi S, Pook MA. PLoS One. 2014 Feb 21;9(2):e89488.

Functional characterization of Friedreich ataxia iPS-derived neuronal progenitors - Functional characterization of Friedreich ataxia iPS-derived neuronal progenitors

Bird MJ, Needham K, Frazier AE, van Rooijen J, Leung J, Hough S, Denham M, Thornton ME, Parish CL, Nayagam BA, Pera M, Thorburn DR, Thompson LH, Dottori M. PLoS One. 2014 Jul 7;9(7):e101718.

Dyclonine rescues frataxin deficiency in animal models and buccal cells of - Dyclonine rescues frataxin deficiency in animal models and buccal cells of

Sahdeo S, Scott BD, McMackin MZ, Jasoliya M, Brown B, Wulff H, Perlman SL, Pook MA, Cortopassi GA. Hum Mol Genet. 2014 Dec 20;23(25):6848-62.

Pharmacological screening using an FXN-EGFP cellular genomic reporter assay for - Pharmacological screening using an FXN-EGFP cellular genomic reporter assay for

Li L, Voullaire L, Sandi C, Pook MA, Ioannou PA, Delatycki MB, Sarsero JP. PLoS One. 2013;8(2):e55940.

Development of frataxin gene expression measures for the evaluation of - Development of frataxin gene expression measures for the evaluation of

Plasterer HL, Deutsch EC, Belmonte M, Egan E, Lynch DR, Rusche JR. PLoS One. 2013 May 17;8(5):e63958.

A GAA repeat expansion reporter model of Friedreich's ataxia recapitulates the - A GAA repeat expansion reporter model of Friedreich's ataxia recapitulates the

Lufino MM, Silva AM, Nemeth AH, Alegre-Abarrategui J, Russell AJ, Wade-Martins R. Hum Mol Genet. 2013 Dec 20;22(25):5173-87.

Blood cells from Friedreich ataxia patients harbor frataxin deficiency without a - Blood cells from Friedreich ataxia patients harbor frataxin deficiency without a

Selak MA, Lyver E, Micklow E, Deutsch EC, Onder O, Selamoglu N, Yager C, Knight S, Carroll M, Daldal F, Dancis A, Lynch DR, Sarry JE. Mitochondrion. 2011 Mar;11(2):342-50.

Mesenchymal stem cells restore frataxin expression and increase hydrogen peroxide - Mesenchymal stem cells restore frataxin expression and increase hydrogen peroxide

Kemp K, Mallam E, Hares K, Witherick J, Scolding N, Wilkins A. PLoS One. 2011;6(10):e26098.