Example standard curve of a serially diluted recombinant HIF1A in the working range of the assay
Sample experiment comparing vehicle and DFO treated HeLa cell lysates. Cell lysates at varying concentrations with the working range of the assay were analyzed.
Demonstration of assay specificity. (A) Western blot using the HIF1A detector antibody (ab51608): lane 1 is vehicle treated HeLa cell lysate and lane 2 is HeLa cell lysate that was treated with deferoxamine (DFO). (B) Raw HIF1A ELISA data using 4mg/mL vehicle or DFO treated HeLa lysate.
Demonstration of assay specificity. Western blot using the HIF1A detector antibody (ab51608): lane 1 is vehicle treated HeLa cell lysate and lane 2 is HeLa cell lysate that was treated with deferoxamine (DFO).