HIF1A

Gene Symbol HIF1A
Entrez Gene 3091
Alt Symbol HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
Species Human
Gene Type protein-coding
Description hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Other Description ARNT interacting protein|ARNT-interacting protein|HIF-1-alpha|PAS domain-containing protein 8|basic-helix-loop-helix-PAS protein MOP1|class E basic helix-loop-helix protein 78|hypoxia-inducible factor 1 alpha isoform I.3|hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|hypoxia-inducible factor 1-alpha|hypoxia-inducible factor1alpha|member of PAS protein 1|member of PAS superfamily 1
Swissprots Q16665 Q53XP6 C0LZJ3 Q9UPB1 Q96PT9
Accessions AAC68568 AAF20149 EAW80806 EAW80807 EAW80808 EAW80809 Q16665 AB073325 BAB70608 AB500182 BAI49183 AB733094 BAM28632 AF207601 AAF20139 AF207602 AAF20140 AF304431 AAG43026 AJ227916 AK093749 AK296876 BAG59438 AK299551 BAG61496 AK304315 BAG65167 AK304436 BAG65259 AK312397 BAG35314 AU123241 BC012527 AAH12527 BT009776 AAP88778 BX648795 CN264295 CN264320 DC344032 DQ975378 FJ790247 ACN88547 U22431 AAC50152 U29165 AAC51210 X72726 NM_001243084 NP_001230013 NM_001530 NP_001521 NM_181054 NP_851397
Function Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. {ECO:0000269|PubMed:11292861, EC
Subcellular Location Cytoplasm. Nucleus. Nucleus speckle {ECO:0000250|UniProtKB:Q61221}. Note=Colocalizes with HIF3A in the nucleus and speckles (By similarity). Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia. {ECO:0000250|UniProtKB:Q61221}.
Tissue Specificity Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. A higher level expression seen in pituitary tumors as compared to the pituitary gland. {ECO:0000269|PubMed:22009797}.
Top Pathways Renal cell carcinoma, Central carbon metabolism in cancer, Choline metabolism in cancer, Proteoglycans in cancer, mTOR signaling pathway

PathScan ® Hydroxy-HIF-1α (Pro564) Sandwich ELISA Kit - 13201 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Treatment of HeLa cells with the hydroxylase inhibitor dimethyloxaloylglycine (DMOG) results in decreased hydroxylation of HIF-1α, as detected by the PathScan® Hydroxy-HIF-1α (Pro564) Sandwich ELISA Kit #13201, but does not affect the level of total HIF-1α detected by PathScan® Total HIF-1α Sandwich ELISA Kit #13127. Absorbance at 450 nm is shown in the top figures while corresponding western blots using a total HIF-1α antibody (left) and Hydroxy-HIF-1α (Pro564) (D43B5) XP® Rabbit mAb #3434 (right) are shown in the bottom figures. Treatment of HeLa cells with the proteasome inhibitor MG-132 #2194 stabilizes HIF-1α protein.

PathScan ® Total HIF-1α Sandwich ELISA Kit - 13127 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)

HIF-1-alpha Human SimpleStep ELISA™ Kit - ab171577 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity 42 pg/ml
Range 0.23 ng/ml - 15 ng/ml

Hif1a + BNIP3 Hypoxia Human In Cell ELISA Kit (IR) - ab129733 from Abcam

Category ELISA Kit
Prices $438.00
Sizes 1 x 96 tests
Sample experiment using ab129733 on HeLa cells treated with a titration of DFO. HeLa cells were seeded to an amine coated 96-well microplate and the following day treated with a titration of DFO. After 24h of DFO exposure, the cells were fixed and stained as described in the protocol and the normalized data is presented here +/- SD (as described in the protocol and data analysis sections). HIF1 alpha results show DFO concentrations ≥10µM induce HIF1A protein levels in a dose dependent manner.

HIF1A Human ELISA Kit - ab117996 from Abcam

Category ELISA Kit
Sensitivity = 50 pg/ml
Range 0.1 ng/ml - 50 ng/ml
Example standard curve of a serially diluted recombinant HIF1A in the working range of the assay

HIF-1 alpha Transcription Factor Assay Kit - ab133104 from Abcam

Category Cellular Assay
Prices $494.00
Sizes 96 tests
HEK293 cells were treated with 1 mM deferoxamine mesylate (DFO) for 1 or 24 hours. 40 micoliters of nuclear lysates (ab113474; corresponding to 4e6 cells) were tested in duplicates (+/- SD).

Human HIF1A / HIF1 Alpha ELISA Kit (Sandwich ELISA) - LS-F4224 from LifeSpan Bioscience

Category ELISA Kit
Prices $650.00
Sizes 1 plate
Sensitivity 0.156 ng/ml
Range 0.156-10 ng/ml

Mouse HIF1A / HIF1 Alpha ELISA Kit (Sandwich ELISA) - LS-F4226 from LifeSpan Bioscience

Category ELISA Kit
Prices $670.00
Sizes 1 plate
Sensitivity 0.156 ng/ml
Range 0.156-10 ng/ml

Rat HIF1A / HIF1 Alpha ELISA Kit (Sandwich ELISA) - LS-F4225 from LifeSpan Bioscience

Category ELISA Kit
Prices $700.00
Sizes 1 plate
Sensitivity 0.156 ng/ml
Range 0.156-10 ng/ml

Pig HIF1A / HIF1 Alpha ELISA Kit (Sandwich ELISA) - LS-F4883 from LifeSpan Bioscience

Category ELISA Kit
Prices $780.00
Sizes 1 plate
Sensitivity 5.5 pg/ml
Range 0.156-10 ng/ml

Mouse/Human/Rat HIF1A / HIF1 Alpha ELISA Kit (DNA-Binding ELISA) - LS-F771 from LifeSpan Bioscience

Category ELISA Kit
Prices $700.00
Sizes 1 plate
Range 0.156-10 ng/ml

Rabbit HIF1A / HIF1 Alpha ELISA Kit (Sandwich ELISA) - LS-F9850 from LifeSpan Bioscience

Category ELISA Kit
Prices $700.00
Sizes 1 plate
Sensitivity 0.156 ng/ml
Range 0.156-10 ng/ml

Mouse HIF1A / HIF1 Alpha ELISA Kit (Sandwich ELISA) - LS-F11634 from LifeSpan Bioscience

Category ELISA Kit
Prices $600.00
Sizes 1 plate
Sensitivity 78 pg/ml
Range 0.156-10 ng/ml

Rat HIF1A / HIF1 Alpha ELISA Kit (Sandwich ELISA) - LS-F11633 from LifeSpan Bioscience

Category ELISA Kit
Prices $600.00
Sizes 1 plate
Sensitivity 7.8
Range 0.156-10 ng/ml

HIF-1 alpha Colorimetric Assay Kit - KA1321 from Novus Biologicals

Category Assay Kit
Prices $659.00
Sizes 1 kit

Rabbit anti-HIF1-alpha Antibody, Affinity Purified - PLA0081 from Sigma-Aldrich

Prices $323.50
Sizes 100 µl
<B>Immunocytochemistry</B><BR/>Rabbit Anti-HIF1-alpha Antibody, Affinity Purified: <B>Cat. No. PLA0081</B>: Detection of Human HIF1 alpha by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells treated with cobalt chloride-200 mcM (left) or mock treatment (right). Antibody: Affinity purified Rabbit Anti-HIF1 alpha (<B>Cat. No. PLA0081</B>) used at a dilution of 1:100 (2 μg/mL). Detection: Red-fluorescent Goat Anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight<SUP>®</SUP> 594 Conjugated.

Goat anti-HIF1-alpha Antibody, Affinity Purified - PLA0304 from Sigma-Aldrich

Prices $323.50
Sizes 100 µl
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Human/Mouse Total HIF-1 alpha DuoSet IC ELISA - DYC1935-2 from R&D Systems

Category ELISA Kit
Prices $329.00, $649.00, $1,395.00
Sizes 1 kit (for 2 Plates), 1 kit (for 5 Plates), 1 pack (for 15 Plates)
Range 125.00 - 8,000 pg/mL

Human/Mouse Total HIF-1 alpha Cell-Based ELISA - KCB1935 from R&D Systems

Category ELISA Kit
Prices $459.00
Sizes 1 kit

Human HIF1a ELISA Kit - abx151808 from Abbexa

Category ELISA Kit
Sensitivity 0.059 ng/ml
Range 0.156 ng/ml - 10 ng/ml

Hypoxia Inducible Factor 1 Alpha, HIF-1a, Human, ELISA Kit - E-EL-H1277 from ARP American Research Products

Category ELISA Kit
Prices $522.00
Sizes 96 t
Sensitivity 0.094
Range 0.156-10ng/mL

Hypoxia Inducible Factor 1 Alpha (HIF1a), Human, ELISA Kit - SEA798Hu from ARP American Research Products

Category ELISA Kit
Prices $650.00
Sizes 96 t
Sensitivity 0.055ng/mL
Range 0.156-10ng/mL

Hypoxia-inducible factor 1-alpha, HIF1A, Human, ELISA Kit - E0798h from ARP American Research Products

Category ELISA Kit
Prices $476.00
Sizes 96 t
Range 0.156-10 ng/mL
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