Flow Cytometry: FOXP3 Flow Assay Kit [NBP2-29484] - Human PBMCs, stimulated with anti-hCD3 were stained with anti-hCD4 FITC and anti-hCD25 PE, followed by fixation, permeabilization, and staining using anti-FOXP3 APC. CD4+ cells were gated and read for hCD25 PE against either APC isotype control (left) or FOXP3 APC (right).