Background-subtracted data values (mean +/- SD) are graphed.
Background-subtracted data values (mean +/- SD) are graphed.
MRC5 cells were grown in 10F HGDMEM media in the presence of 10 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 ng/mL IL-1 alpha (ab9616) for 48 hours. IL-11 concentrations were measured in serial dilutions of cell culture supernatants using this kit. Background-subtracted data values (mean +/- SD, n = 2) are graphed.
MRC5 cells were grown in 10F HGDMEM media in the absence or presence of 10 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 ng/mL IL-1 alpha (ab9616) for 48 hours. IL-11 concentrations were measured in 8 and 16 X diluted cell culture supernatants using this kit. Interpolated data values adjusted for sample dilution are graphed (mean +/- SD, n=3) in ng of IL-11 per mL of cell culture supernatant.