ImmunocytochemistryRabbit Anti-HIF1-alpha Antibody, Affinity Purified:
Cat. No. PLA0081: Detection of Human HIF1 alpha by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells treated with cobalt chloride-200 mcM (left) or mock treatment (right). Antibody: Affinity purified Rabbit Anti-HIF1 alpha (
Cat. No. PLA0081) used at a dilution of 1:100 (2 μg/mL). Detection: Red-fluorescent Goat Anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight
® 594 Conjugated.
Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).