Rabbit anti-PLK1 Antibody, Affinity Purified

Name	Rabbit anti-PLK1 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0073
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Rabbit, Marmoset, Human, Ape, Chimpanzee
Gene	PLK1
Supplier Page	Shop

Product images

<B>Immunocytochemistry</B><BR/>Rabbit Anti-PLK1 Antibody, Affinity Purified: <B>Cat. No. PLA0073</B>: Detection of Human PLK1 by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified Rabbit Anti-PLK1 (Cat. No.<B>PLA0073</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: Green fluorescent goat Anti-Rabbit IgG highly cross-adsorbed Antibody FITC conjugated used at a dilution of 1:100.

Immunocytochemistry
Rabbit Anti-PLK1 Antibody, Affinity Purified: Cat. No. PLA0073: Detection of Human PLK1 by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified Rabbit Anti-PLK1 (Cat. No.PLA0073) used at a dilution of 1:1,000 (1 μg/mL). Detection: Green fluorescent goat Anti-Rabbit IgG highly cross-adsorbed Antibody FITC conjugated used at a dilution of 1:100.

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-PLK1 Antibody, Affinity Purified: <B>Cat. No. PLA0073</B>: Detection of Human PLK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (50 μg for A; 600 μg for B) from HeLa cells. Antibodies: A. Affinity purified Rabbit Anti-PLK1 Antibody (<B>Cat. No. PLA0073</B>) used at the indicated concentrations for WB. B. Affinity purified Rabbit Anti-PLK1 Antibodies recognizing different epitopes used at 2 μg/600 μg lysate for IP. Immunoprecipitated PLK1 was detected using Antibodies at 0.5 μg/mL. Detection: Chemiluminescence with exposure times of less than 5 minutes.

Immunoblotting
Rabbit Anti-PLK1 Antibody, Affinity Purified: Cat. No. PLA0073: Detection of Human PLK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (50 μg for A; 600 μg for B) from HeLa cells. Antibodies: A. Affinity purified Rabbit Anti-PLK1 Antibody (Cat. No. PLA0073) used at the indicated concentrations for WB. B. Affinity purified Rabbit Anti-PLK1 Antibodies recognizing different epitopes used at 2 μg/600 μg lysate for IP. Immunoprecipitated PLK1 was detected using Antibodies at 0.5 μg/mL. Detection: Chemiluminescence with exposure times of less than 5 minutes.