Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-CDK7 Antibody, Affinity Purified:
Cat. No. PLA0103: Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 μg/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified Rabbit Anti-CDK7 Antibody (
Cat. No. PLA0103) used at 0.1 μg/mL for WB and at 4 μg/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.
ImmunoblottingRabbit Anti-CDK7 Antibody, Affinity Purified:
Cat. No. PLA0103: Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified Rabbit Anti-CDK7 Antibody
PLA0103 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.