Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingGoat Anti-H2AX Antibody, Affinity Purified:
Cat. No. PLA0294: Detection of Human H2AX by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg) cells. Antibodies: Affinity purified goat Anti-H2AX Antibody
PLA0294 used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.