Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-H2AX Antibody, Affinity Purified:
Cat. No. PLA0023: Detection of Human and Mouse Histone H2AX by Western Blot. Samples: Nuclear extract from Human 293T, Human G-361, and wild-type (+/+) or H2AX knockout (-/-) Mouse embryonic fibroblasts. Antibody: Affinity purified Rabbit Anti-H2AX Antibody
PLA0023 used at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 5 seconds.