Rabbit anti-VCP Antibody, Affinity Purified

Name	Rabbit anti-VCP Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0120
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Human, Mouse, Zebrafish, Chicken, Rabbit, Rat, Bovine, Primate, Dog, Horse, Pig, Guinea Pig
Gene	VCP
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-VCP Antibody, Affinity Purified: <B>Cat. No. PLA0120</B>: Detection of Human and Mouse VCP by Immunohistochemistry. Sample: FFPE sections of Human ovarian carcinoma (upper) and Mouse squamous cell carcinoma (lower). Antibody: Affinity purified Rabbit Anti-VCP (<B>Cat. No. PLA0120</B>) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-VCP Antibody, Affinity Purified: Cat. No. PLA0120: Detection of Human and Mouse VCP by Immunohistochemistry. Sample: FFPE sections of Human ovarian carcinoma (upper) and Mouse squamous cell carcinoma (lower). Antibody: Affinity purified Rabbit Anti-VCP (Cat. No. PLA0120) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-VCP Antibody, Affinity Purified: <B>Cat. No. PLA0120</B>: Detection of Human and Mouse VCP by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 1/4 of IP loaded), 293T (Lane T, 50 μg for WB) and Mouse NIH3T3 (Lane M, 50 μg for WB) cells. Antibodies: Affinity purified Rabbit Anti-VCP Antibody (<B>Cat. No. PLA0120</B>) used for WB at 0.04 μg/mL (A) and 1 μg/mL (B), and used in IP at 3 μg/mg lysate (B). VCP was also immunoprecipitated using Rabbit Anti-VCP Antibodies that recognize different epitopes at 3 μg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Immunoblotting
Rabbit Anti-VCP Antibody, Affinity Purified: Cat. No. PLA0120: Detection of Human and Mouse VCP by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 1/4 of IP loaded), 293T (Lane T, 50 μg for WB) and Mouse NIH3T3 (Lane M, 50 μg for WB) cells. Antibodies: Affinity purified Rabbit Anti-VCP Antibody (Cat. No. PLA0120) used for WB at 0.04 μg/mL (A) and 1 μg/mL (B), and used in IP at 3 μg/mg lysate (B). VCP was also immunoprecipitated using Rabbit Anti-VCP Antibodies that recognize different epitopes at 3 μg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).