Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-SMC2 Antibody, Affinity Purified:
Cat. No. PLA0018: Detection of Human SMC2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (90 μg for WB; 0.5 mg for IP) from HEK293T cells that were mock transfected (E), transfected with full length SMC2 (T for WB), or transfected with truncated SMC2 (T for IP). Antibody: Affinity purified Rabbit Anti-SMC2
PLA0018 used at 0.1 μg/mL for WB and at 5 μg for IP. Detection: Chemiluminescence.