Goat anti-SMC1 Antibody, Affinity Purified

Name	Goat anti-SMC1 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0291
Prices	$323.50
Sizes	100 µl
Applications	IP
Species Reactivities	Rat, Chicken, Rabbit, Guinea Pig, Bovine, Human, Monkey, Horse, Pig, Mouse, Dog, Primate
Gene	SMC1A
Supplier Page	Shop

Product images

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Goat Anti-SMC1 Antibody, Affinity Purified: <B>Cat. No. PLA0291</B>: Detection of Human SMC1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (Lane H; 50 μg), and Jurkat (Lane J; 50 μg) cells. Antibodies: Affinity purified goat Anti-SMC1 Antibody (<B>Cat. No. PLA0291</B>) used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Goat Anti-SMC1 Antibody, Affinity Purified: Cat. No. PLA0291: Detection of Human SMC1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (Lane H; 50 μg), and Jurkat (Lane J; 50 μg) cells. Antibodies: Affinity purified goat Anti-SMC1 Antibody (Cat. No. PLA0291) used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

<B>Immunoblotting</B><BR/>Goat Anti-SMC1 Antibody, Affinity Purified: <B>Cat. No. PLA0291</B>: Detection of Mouse SMC1 by Western Blot. Samples: Whole cell lysate (15 and 50 μg) from NIH3T3 cells. Antibodies: Affinity purified goat Anti-SMC1 Antibody (<B>Cat. No. PLA0291</B>) used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoblotting
Goat Anti-SMC1 Antibody, Affinity Purified: Cat. No. PLA0291: Detection of Mouse SMC1 by Western Blot. Samples: Whole cell lysate (15 and 50 μg) from NIH3T3 cells. Antibodies: Affinity purified goat Anti-SMC1 Antibody (Cat. No. PLA0291) used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

<B>Immunoprecipitation</B><BR/>Goat Anti-SMC1 Antibody, Affinity Purified: <B>Cat. No. PLA0291</B>: Detection of Human SMC1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified goat Anti-SMC1 antibody <B>Cat. No. PLA0291</B> used for IP at 6 μg/mg lysate. SMC1 was also immunoprecipitated by Rabbit Anti-SMC1 antibody <B>PLA0017</B>. For blotting immunoprecipitated SMC1, PLA0291 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation
Goat Anti-SMC1 Antibody, Affinity Purified: Cat. No. PLA0291: Detection of Human SMC1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified goat Anti-SMC1 antibody Cat. No. PLA0291 used for IP at 6 μg/mg lysate. SMC1 was also immunoprecipitated by Rabbit Anti-SMC1 antibody PLA0017. For blotting immunoprecipitated SMC1, PLA0291 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.