Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-TSG101 Antibody, Affinity Purified:
Cat. No. PLA0283: Detection of Human and Mouse TSG101 by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 μg), 293T (Lane T; 50 μg), Jurkat (Lane J; 50 μg) and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-TSG101 Antibody
PLA0283 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.