Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-Rad50 Antibody, Affinity Purified:
Cat. No. PLA0058: Detection of Human Rad50 by Immunoprecipitation and Western Blot. Sample: Nuclear extract (1 mg) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-Rad50 (
Cat. No. PLA0058) used at 0.25 μg/mL for WB of Rad50 immunoprecipitated by 1.25 μg of affinity purified Rabbit Anti-Rad50 or affinity purified Rabbit Anti-Rad50, recognizing a different epitope. Detection: Chemiluminescence.