Rabbit anti-SMC3 Antibody, Affinity Purified

Name	Rabbit anti-SMC3 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0019
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Rabbit, Horse, Pig, Mouse, Turkey, Bovine, Primate, Rat, Human, Chicken, Dog, Guinea Pig
Gene	SMC3
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-SMC3 Antibody, Affinity Purified: <B>Cat. No. PLA0019</B>: Detection of Human SMC3 by Immunohistochemistry. Sample: FFPE section of Human testicular seminoma. Antibody: Affinity purified Rabbit Anti-SMC3 (<B>Cat. No. PLA0019</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-SMC3 Antibody, Affinity Purified: Cat. No. PLA0019: Detection of Human SMC3 by Immunohistochemistry. Sample: FFPE section of Human testicular seminoma. Antibody: Affinity purified Rabbit Anti-SMC3 (Cat. No. PLA0019) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-SMC3 Antibody, Affinity Purified: <B>Cat. No. PLA0019</B>: Detection of Human and Mouse SMC3 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, Jurkat, Mouse TCMK-1, and Mouse NIH3T3 cells. Antibodies: Affinity purified Rabbit Anti-SMC3 Antibody <B>PLA0019</B> used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunoblotting
Rabbit Anti-SMC3 Antibody, Affinity Purified: Cat. No. PLA0019: Detection of Human and Mouse SMC3 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, Jurkat, Mouse TCMK-1, and Mouse NIH3T3 cells. Antibodies: Affinity purified Rabbit Anti-SMC3 Antibody PLA0019 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-SMC3 Antibody, Affinity Purified: <B>Cat. No. PLA0019</B>: Detection of Human SMC3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-SMC3 antibody <B>Cat. No. PLA0019</B> used for IP at 6 μg per reaction. SMC3 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated SMC3, <B>PLA0019</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunoprecipitation
Rabbit Anti-SMC3 Antibody, Affinity Purified: Cat. No. PLA0019: Detection of Human SMC3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-SMC3 antibody Cat. No. PLA0019 used for IP at 6 μg per reaction. SMC3 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated SMC3, PLA0019 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.