Goat anti-CRSP1/TRAP220 Antibody, Affinity Purified

Name	Goat anti-CRSP1/TRAP220 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0300
Prices	$323.50
Sizes	100 µl
Applications	IP
Species Reactivities	Mouse, Human
Gene	MED1
Supplier Page	Shop

Product images

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Goat Anti-CRSP1/TRAP220 Antibody, Affinity Purified: <B>Cat. No. PLA0300</B>: Detection of Human and Mouse CRSP1/TRAP220 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (50 μg), Jurkat (50 μg), and Mouse NIH3T3 (50 μg) cells. Antibodies: Affinity purified goat Anti-CRSP1/TRAP220 Antibody <B>PLA0300</B> used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Goat Anti-CRSP1/TRAP220 Antibody, Affinity Purified: Cat. No. PLA0300: Detection of Human and Mouse CRSP1/TRAP220 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (50 μg), Jurkat (50 μg), and Mouse NIH3T3 (50 μg) cells. Antibodies: Affinity purified goat Anti-CRSP1/TRAP220 Antibody PLA0300 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

<B>Immunoprecipitation</B><BR/>Goat Anti-CRSP1/TRAP220 Antibody, Affinity Purified: <B>Cat. No. PLA0300</B>: Detection of Human CRSP1/TRAP220 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat Anti-CRSP1/TRAP220 antibody <B>Cat. No. PLA0300</B> used for IP at 6 μg/mg lysate. CRSP1/TRAP220 was also immunoprecipitated by Rabbit Anti-CRSP1/TRAP220 antibody <B>Cat. No. PLA0142</B>. For blotting immunoprecipitated CRSP1/TRAP220, PLA0142 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation
Goat Anti-CRSP1/TRAP220 Antibody, Affinity Purified: Cat. No. PLA0300: Detection of Human CRSP1/TRAP220 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat Anti-CRSP1/TRAP220 antibody Cat. No. PLA0300 used for IP at 6 μg/mg lysate. CRSP1/TRAP220 was also immunoprecipitated by Rabbit Anti-CRSP1/TRAP220 antibody Cat. No. PLA0142. For blotting immunoprecipitated CRSP1/TRAP220, PLA0142 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.