Rabbit anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified

Name	Rabbit anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0141
Prices	$345.00
Sizes	100 µl
Applications	FC
Species Reactivities	Rat, Pig, Horse, Human, Rabbit, Amphibians, Dog, Mouse, Bovine, Guinea Pig
Gene	MCM2
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified: <B>Cat. No. PLA0141</B>: Detection of Human Phospho MCM2 (S40/S41) by Immunohistochemistry. Samples: FFPE serial sections of Human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified Rabbit Anti-Phospho MCM2 (S40/S41) (<B>Cat. No. PLA0141</B>) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified: Cat. No. PLA0141: Detection of Human Phospho MCM2 (S40/S41) by Immunohistochemistry. Samples: FFPE serial sections of Human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified Rabbit Anti-Phospho MCM2 (S40/S41) (Cat. No. PLA0141) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified: <B>Cat. No. PLA0141</B>: Detection of Human Phospho MCM2 (S40/S41) by Western Blot. Samples: Whole cell lysate (50 μg) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody: Affinity purified Rabbit Anti-phospho MCM2 (S40/S41) Antibody (<B>Cat. No. PLA0141</B>) used at 0.1 μg/mL. To examine total MCM2, the blot was stripped and then blotted with Rabbit Anti-MCM2 Antibody (<B>Cat. No. PLA0060</B>) at 0.1 μg/mL. Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).

Immunoblotting
Rabbit Anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified: Cat. No. PLA0141: Detection of Human Phospho MCM2 (S40/S41) by Western Blot. Samples: Whole cell lysate (50 μg) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody: Affinity purified Rabbit Anti-phospho MCM2 (S40/S41) Antibody (Cat. No. PLA0141) used at 0.1 μg/mL. To examine total MCM2, the blot was stripped and then blotted with Rabbit Anti-MCM2 Antibody (Cat. No. PLA0060) at 0.1 μg/mL. Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).

<B>Flow Cytometry</B><BR/>Rabbit Anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified: <B>Cat. No. PLA0141</B>: Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 × 10<SUP>6</SUP> cells were stained with 0.1 μg/mL Anti-phospho-MCM2 (pS40/41) [<B>PLA0141</B>] in a 150 mcl volume. DNA content was simultaneously analyzed via PI stain.

Flow Cytometry
Rabbit Anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified: Cat. No. PLA0141: Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 × 10⁶ cells were stained with 0.1 μg/mL Anti-phospho-MCM2 (pS40/41) [PLA0141] in a 150 mcl volume. DNA content was simultaneously analyzed via PI stain.