Rabbit anti-Phospho MCM2 (S108) Antibody, Affinity Purified

Name	Rabbit anti-Phospho MCM2 (S108) Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0026
Prices	$345.00
Sizes	100 µl
Applications	FC
Species Reactivities	Rat, Horse, Pig, Mouse, Dog, Bovine, Primate, Chicken, Turkey, Rabbit, Human, Guinea Pig
Gene	MCM2
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-Phospho MCM2 (S108) Antibody, Affinity Purified: <B>Cat. No. PLA0026</B>: Detection of Human and Mouse Phospho MCM2 (S108) by Immunohistochemistry. Sample: FFPE sections of Human breast carcinoma (left) and Mouse renal cell carcinoma (right). Antibody: Affinity purified Rabbit Anti-phospho MCM2 (S108) (<B>Cat. No. PLA0026</B>) used at a dilution of 1:5,000 (0.2 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-Phospho MCM2 (S108) Antibody, Affinity Purified: Cat. No. PLA0026: Detection of Human and Mouse Phospho MCM2 (S108) by Immunohistochemistry. Sample: FFPE sections of Human breast carcinoma (left) and Mouse renal cell carcinoma (right). Antibody: Affinity purified Rabbit Anti-phospho MCM2 (S108) (Cat. No. PLA0026) used at a dilution of 1:5,000 (0.2 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-Phospho MCM2 (S108) Antibody, Affinity Purified: <B>Cat. No. PLA0026</B>: Detection of Human and Mouse Phospho MCM2 (S108) by Western Blot. Samples: Whole cell lysate (15 or 50 μg) from 293T cells or Mouse NIH3T3 (M) cells. Cells were either mock treated (-) or treated (+) with etoposide (100 μM, 2 hours). Antibodies: Affinity purified Rabbit Anti-phospho MCM2 (S108) Antibody <B>PLA0026</B> used for WB at 0.1 mcg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunoblotting
Rabbit Anti-Phospho MCM2 (S108) Antibody, Affinity Purified: Cat. No. PLA0026: Detection of Human and Mouse Phospho MCM2 (S108) by Western Blot. Samples: Whole cell lysate (15 or 50 μg) from 293T cells or Mouse NIH3T3 (M) cells. Cells were either mock treated (-) or treated (+) with etoposide (100 μM, 2 hours). Antibodies: Affinity purified Rabbit Anti-phospho MCM2 (S108) Antibody PLA0026 used for WB at 0.1 mcg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-Phospho MCM2 (S108) Antibody, Affinity Purified: <B>Cat. No. PLA0026</B>: Detection of Human Phospho MCM2 (S108) by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells either mock treated (-) or treated (+) with etoposide (100 μM, 2 hours). Antibodies: MCM2 was immunoprecipitated using Rabbit Anti-MCM2 antibody <B>Cat. No. PLA0060</B>. Following immunoprecipitation the product of the IP reaction was either mock treated (-) or treated with phosphatases (+). Affinity purified Rabbit Anti-phospho MCM2 (S108) antibody <B>Cat. No. PLA0026</B> was used for WB at 1 mcg/mL. To examine total MCM2, the blot was stripped and then blotted with Rabbit Anti-MCM2 antibody <B>PLA0060</B>. Detection: Chemiluminescence with exposure times of 10 seconds (upper and lower panels).

Immunoprecipitation
Rabbit Anti-Phospho MCM2 (S108) Antibody, Affinity Purified: Cat. No. PLA0026: Detection of Human Phospho MCM2 (S108) by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells either mock treated (-) or treated (+) with etoposide (100 μM, 2 hours). Antibodies: MCM2 was immunoprecipitated using Rabbit Anti-MCM2 antibody Cat. No. PLA0060. Following immunoprecipitation the product of the IP reaction was either mock treated (-) or treated with phosphatases (+). Affinity purified Rabbit Anti-phospho MCM2 (S108) antibody Cat. No. PLA0026 was used for WB at 1 mcg/mL. To examine total MCM2, the blot was stripped and then blotted with Rabbit Anti-MCM2 antibody PLA0060. Detection: Chemiluminescence with exposure times of 10 seconds (upper and lower panels).