Rabbit anti-MCM2 Antibody, Affinity Purified

Name	Rabbit anti-MCM2 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0060
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Mouse, Primate, Human, Ape, Chimpanzee, Amphibians
Gene	MCM2
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-MCM2 Antibody, Affinity Purified: <B>Cat. No. PLA0060</B>: Detection of Human MCM2 by Immunohistochemistry. Sample: FFPE section of Human small cell lung cancer. Antibody: Affinity purified Rabbit Anti-MCM2 (<B>Cat. No. PLA0060</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-MCM2 Antibody, Affinity Purified: Cat. No. PLA0060: Detection of Human MCM2 by Immunohistochemistry. Sample: FFPE section of Human small cell lung cancer. Antibody: Affinity purified Rabbit Anti-MCM2 (Cat. No. PLA0060) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-MCM2 Antibody, Affinity Purified: <B>Cat. No. PLA0060</B>: Detection of Human MCM2 by Western Blot and Immunoprecipitation. Samples: RIPA extract (50 μg for WB; 7 mg for CoIP) from HeLa cells. Antibody: Affinity purified Rabbit Anti-MCM2 (<B>Cat. No. PLA0060</B>) used at 0.2 μg/mL for WB and 20 μg/7 mg extract for IP. Detection: Chemiluminescence for WB; Coomassie staining for CoIP. Identity of CoIP proteins determined by mass spectrometry.

Immunoblotting
Rabbit Anti-MCM2 Antibody, Affinity Purified: Cat. No. PLA0060: Detection of Human MCM2 by Western Blot and Immunoprecipitation. Samples: RIPA extract (50 μg for WB; 7 mg for CoIP) from HeLa cells. Antibody: Affinity purified Rabbit Anti-MCM2 (Cat. No. PLA0060) used at 0.2 μg/mL for WB and 20 μg/7 mg extract for IP. Detection: Chemiluminescence for WB; Coomassie staining for CoIP. Identity of CoIP proteins determined by mass spectrometry.

<B>Immunoblotting</B><BR/>Rabbit Anti-MCM2 Antibody, Affinity Purified: <B>Cat. No. PLA0060</B>: Detection of Mouse MCM2 by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified Rabbit Anti-MCM2 Antibody <B>PLA0060</B> used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoblotting
Rabbit Anti-MCM2 Antibody, Affinity Purified: Cat. No. PLA0060: Detection of Mouse MCM2 by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified Rabbit Anti-MCM2 Antibody PLA0060 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.