Rabbit anti-ErbB2 Antibody, Affinity Purified

Name	Rabbit anti-ErbB2 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0123
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Mouse, Primate, Ape, Chimpanzee, Monkey, Dog, Horse, Rabbit, Pig, Human, Rat
Gene	ERBB2
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-ErbB2 Antibody, Affinity Purified: <B>Cat. No. PLA0123</B>: Detection of Human ErbB2 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-ErbB2 (<B>Cat. No. PLA0123</B>) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-ErbB2 Antibody, Affinity Purified: Cat. No. PLA0123: Detection of Human ErbB2 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-ErbB2 (Cat. No. PLA0123) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-ErbB2 Antibody, Affinity Purified: <B>Cat. No. PLA0123</B>: Detection of Human and Mouse ErbB2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded), 293T (Lane T; 50 μg for WB) and Mouse NIH3T3 (Lane M; 50 μg for WB) cells. Antibodies: Affinity purified Rabbit Anti-ErbB2 Antibody (<B>Cat. No. PLA0123</B>) used for WB at 1 μg/mL (A) and 0.04 μg/mL (B) and used for IP at 3 μg/mg lysate. ErbB2 was also immunoprecipitated using Rabbit Anti-ErbB2 Antibodies recognizing different epitopes at 3 μg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Immunoblotting
Rabbit Anti-ErbB2 Antibody, Affinity Purified: Cat. No. PLA0123: Detection of Human and Mouse ErbB2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded), 293T (Lane T; 50 μg for WB) and Mouse NIH3T3 (Lane M; 50 μg for WB) cells. Antibodies: Affinity purified Rabbit Anti-ErbB2 Antibody (Cat. No. PLA0123) used for WB at 1 μg/mL (A) and 0.04 μg/mL (B) and used for IP at 3 μg/mg lysate. ErbB2 was also immunoprecipitated using Rabbit Anti-ErbB2 Antibodies recognizing different epitopes at 3 μg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).