Rabbit anti-Phospho Rad17 (S645) Antibody, Affinity Purified

Name	Rabbit anti-Phospho Rad17 (S645) Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0052
Prices	$345.00
Sizes	100 µl
Applications	PLA
Species Reactivities	Ape, Primate, Monkey, Marmoset, Chimpanzee, Human
Gene	RAD17
Supplier Page	Shop

Product images

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-Phospho Rad17 (S645) Antibody, Affinity Purified: <B>Cat. No. PLA0052</B>: Detection of Human Rad17, Phospho (S645) by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa and 293T cells treated with 100 μM etoposide for 2 hours (+) or mock treated (-). Antibodies: Affinity purified Rabbit Anti-Rad17, Phospho (S645) Antibody <B>PLA0052</B> used for WB at 0.4 μg/mL. To examine total Rad17, the membrane was reblotted with goat Anti-Rad17 Antibody <B>PLA0051</B> (lower panel). Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).

Immunoblotting
Rabbit Anti-Phospho Rad17 (S645) Antibody, Affinity Purified: Cat. No. PLA0052: Detection of Human Rad17, Phospho (S645) by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa and 293T cells treated with 100 μM etoposide for 2 hours (+) or mock treated (-). Antibodies: Affinity purified Rabbit Anti-Rad17, Phospho (S645) Antibody PLA0052 used for WB at 0.4 μg/mL. To examine total Rad17, the membrane was reblotted with goat Anti-Rad17 Antibody PLA0051 (lower panel). Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).