Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-Phospho HdmX/MDM4 (S403) Antibody, Affinity Purified:
Cat. No. PLA0102: Detection of phospho HdmX/MDM4 (Ser 403) by Western Blot. Samples: Whole cell lysate (30 μg/lane) from 293T cells that were mock transfected, or transfected with expression constructs for HA tagged wild-type HdmX/MDM4 or HA tagged HdmX/MDM4 (S403A) mutant. Antibody: Affinity purified Rabbit Anti-phospho HdmX/MDM4 Antibody (
Cat. No. PLA0102) used at 0.13 μg/mL for WB. Detection: Chemiluminescence with an exposure time of 30 seconds.