Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-PARP1 Antibody, Affinity Purified:
Cat. No. PLA0184: Detection of Human PARP1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 μg) from 293T cells. Antibody: Affinity purified Rabbit Anti-PARP1 Antibody
PLA0184 used for WB at 0.04 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.