Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).
ImmunoblottingRabbit Anti-DNMT1 Antibody, Affinity Purified:
Cat. No. PLA0011: Detection of Human DNMT1 by Western Blot and Immunoprecipitation. Samples: Nuclear extract [50 μg/lane for A; 10 μg (input) or 1 mg (IP) for B] from HeLa cells. Antibodies: A. Affinity purified Rabbit Anti-DNMT1 Antibody
PLA0011 used at the indicated concentrations for WB; B. DNMT1 was immunoprecipitated with affinity purified Rabbit Anti-DNMT1 Antibody
PLA0011 then detected with a different affinity purified Rabbit Anti-DNMT1 Antibody used at 0.2 μg/mL for WB. Detection: Chemiluminescence with an exposure time of 2 seconds.