IL-1Ra measured in lysates from control cells (-) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours (IL-1Ra per mg of extracted protein; duplicates +/- SD).
IL-1Ra measured in culture supernatants from control (-) or activated PBMCs ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) and in human biological fluids. Signals from plasma and serum were below level of detection (duplicates +/- SD).
Standard curve with background signal subtracted (duplicates; +/- SD).
Representative standard curve using ab100565