Rabbit anti-Cul4a Antibody, Affinity Purified

Name	Rabbit anti-Cul4a Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0136
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Dog, Horse, Chimpanzee, Human, Rabbit, Monkey, Mouse, Turkey, Chicken, Ape
Gene	CUL4A
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-Cul4a Antibody, Affinity Purified: <B>Cat. No. PLA0136</B>: Detection of Human and Mouse Cul4a by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma (left) and Mouse squamous cell carcinoma (right). Antibody: Affinity purified Rabbit Anti-Cul4a (<B>Cat. No. PLA0136</B>) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-Cul4a Antibody, Affinity Purified: Cat. No. PLA0136: Detection of Human and Mouse Cul4a by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma (left) and Mouse squamous cell carcinoma (right). Antibody: Affinity purified Rabbit Anti-Cul4a (Cat. No. PLA0136) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-Cul4a Antibody, Affinity Purified: <B>Cat. No. PLA0136</B>: Detection of Human and Mouse Cul4a by WB and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded), 293T (Lane T; 50 μg), and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-Cul4a Antibody (<B>Cat. No. PLA0136</B>) used for WB at 0.1 μg/mL (A) and 1 μg/mL (B) and used for IP at 3 μg/mg lysate (B). Cul4a was also immunoprecipitated by a Rabbit Anti-Cul4a Antibody recognizing a different epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

Immunoblotting
Rabbit Anti-Cul4a Antibody, Affinity Purified: Cat. No. PLA0136: Detection of Human and Mouse Cul4a by WB and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded), 293T (Lane T; 50 μg), and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-Cul4a Antibody (Cat. No. PLA0136) used for WB at 0.1 μg/mL (A) and 1 μg/mL (B) and used for IP at 3 μg/mg lysate (B). Cul4a was also immunoprecipitated by a Rabbit Anti-Cul4a Antibody recognizing a different epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).