Goat anti-RNA Polymerase II Antibody, Affinity Purified

Name	Goat anti-RNA Polymerase II Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0292
Prices	$323.50
Sizes	100 µl
Applications	IP
Species Reactivities	Zebrafish, Mouse, Bovine, Monkey, Pig, Primate, Rabbit, Human, Ape, Amphibians, Rat, Dog
Gene	POLR2A
Supplier Page	Shop

Product images

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Goat Anti-RNA Polymerase II Antibody, Affinity Purified: <B>Cat. No. PLA0292</B>: Detection of Human RNA Polymerase II by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (Lane H; 50 μg), and Jurkat (Lane J; 50 μg) cells. Antibodies: Affinity purified goat Anti-RNA Polymerase II Antibody (<B>Cat. No. PLA0292</B>) used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoblotting
Goat Anti-RNA Polymerase II Antibody, Affinity Purified: Cat. No. PLA0292: Detection of Human RNA Polymerase II by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (Lane H; 50 μg), and Jurkat (Lane J; 50 μg) cells. Antibodies: Affinity purified goat Anti-RNA Polymerase II Antibody (Cat. No. PLA0292) used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

<B>Immunoblotting</B><BR/>Goat Anti-RNA Polymerase II Antibody, Affinity Purified: <B>Cat. No. PLA0292</B>: Detection of Mouse RNA Polymerase II by Western Blot. Samples: Whole cell lysate (15 and 50 μg) from NIH3T3 cells. Antibodies: Affinity purified goat Anti-RNA Polymerase II Antibody (<B>Cat. No. PLA0292</B>) used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoblotting
Goat Anti-RNA Polymerase II Antibody, Affinity Purified: Cat. No. PLA0292: Detection of Mouse RNA Polymerase II by Western Blot. Samples: Whole cell lysate (15 and 50 μg) from NIH3T3 cells. Antibodies: Affinity purified goat Anti-RNA Polymerase II Antibody (Cat. No. PLA0292) used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

<B>Immunoprecipitation</B><BR/>Goat Anti-RNA Polymerase II Antibody, Affinity Purified: <B>Cat. No. PLA0292</B>: Detection of Human RNA Polymerase II by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat Anti-RNA Polymerase II antibody <B>Cat. No. PLA0292</B> used for IP at 6 μg/mg lysate. For blotting immunoprecipitated RNA Polymerase II, <B>PLA0292</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation
Goat Anti-RNA Polymerase II Antibody, Affinity Purified: Cat. No. PLA0292: Detection of Human RNA Polymerase II by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat Anti-RNA Polymerase II antibody Cat. No. PLA0292 used for IP at 6 μg/mg lysate. For blotting immunoprecipitated RNA Polymerase II, PLA0292 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.