POLR2A

Gene Symbol POLR2A
Entrez Gene 5430
Alt Symbol POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1
Species Human
Gene Type protein-coding
Description polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Other Description DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit|DNA-directed RNA polymerase II subunit A|DNA-directed RNA polymerase II subunit RPB1|DNA-directed RNA polymerase III largest subunit|RNA polymerase II subunit B1|RNA-directed RNA polymerase II subunit RPB1
Swissprots A6NN93 Q6NX41 B9EH88 P24928
Accessions CAA52862 EAW90181 EAW90182 EAW90183 P24928 AK293777 BAG57192 AK304882 BAG65619 AW589665 BC067295 AAH67295 BC107140 BC137231 AAI37232 BQ353795 BQ891728 BU730469 BU739765 CV355924 DA923649 M21610 AAA60135 X63564 CAA45125 NM_000937 NP_000928
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA h
Subcellular Location Nucleus {ECO:0000269|PubMed:9852112}.
Top Pathways Huntington's disease, Herpes simplex infection, Epstein-Barr virus infection, Purine metabolism, RNA polymerase

Mouse/Human/Rat POLR2A / RNA polymerase II ELISA Kit (Cell-Based ELISA) - LS-F2264 from LifeSpan Bioscience

Category ELISA Kit
Prices $450.00
Sizes 1 plate
<B>Immunohistochemistry</B><BR/>Rabbit Anti-Phospho RNA Polymerase II (S2) Antibody, Affinity Purified: <B>Cat. No. PLA0128</B>: Detection of Human Phospho-RNA Polymerase II (S2) by Immunohistochemistry. Samples: FFPE serial sections of Human ovarian carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified Rabbit Anti-Phospho-RNA Polymerase II (S2) (<B>Cat. No. PLA0128</B>) used at a dilution of 1:500 ( 0.4 μg/mL). Detection: DAB

Goat anti-RNA Polymerase II Antibody, Affinity Purified - PLA0292 from Sigma-Aldrich

Prices $323.50
Sizes 100 µl
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Rabbit anti-RNA Polymerase II Antibody, Affinity Purified - PLA0127 from Sigma-Aldrich

Prices $323.50
Sizes 100 µl
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

DNA-directed RNA polymerase II subunit RPB1 (POLR2A), Human, ELISA Kit - CSB-EL018327HU from ARP American Research Products

Category ELISA Kit
Prices $495.00, $695.00, $696.00, $699.00
Sizes 48 t, 96 t, 5 x 96t, 10 x 96t
Sensitivity 6.25 pg/ml
Range 25 pg/ml-1600 pg/ml

POLR2A Colorimetric Cell-Based ELISA Kit - CB5580 from Assay Biotechnology

Category ELISA Kit
Prices $380.00, $760.00, $1,140.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

POLR2A (Phospho-Ser1619) Colorimetric Cell-Based ELISA Kit - CBP1075 from Assay Biotechnology

Category ELISA Kit
Prices $480.00, $960.00, $1,440.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, ELISA Kit - MBS943991 from MyBioSource

Category ELISA Kit
Prices $250.00, $495.00, $710.00, $2,705.00, $5,145.00
Sizes 24 -strip-wells (Limit 1), 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity The minimum detectable dose of human POLR2A is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Range 25 pg/ml -1600 pg/ml.

Human DNA-directed RNA polymerase II subunit RPB1(POLR2A) ELISA kit - CSB-EL018327HU from Cusabio

Category ELISA Kit
Sensitivity 6.25 pg/ml.
Range 25 pg/ml-1600 pg/ml

POLR2A ELISA Kit - DEIA-XYA1493 from Creative Diagnostics

Category ELISA Kit

POLR2A (Phospho-Ser1619) ELISA Kit - DEIA-XYA1494 from Creative Diagnostics

Category ELISA Kit

Human DNA-directed RNA polymerase II subunit RPB1 (POLR2A) ELISA Kit - EKC33496 from Biomatik

Category ELISA Kit
Prices $705.00, $2,820.00, $5,288.00
Sizes 96 t, 5x96t, 10x96t
Sensitivity 6.25 pg/ml
Range 25 pg/ml-1600 pg/ml

Human POLR2A ELISA Kit - E030113 from Biobool

Category ELISA Kit
Sensitivity The minimum detectable dose of human POLR2A is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m

POLR2A ELISA Kit (Human) (OKCA00661) - OKCA00661 from Aviva Systems Biology

Category ELISA Kit
Prices $590.00
Sizes 96 wells
Sensitivity 6.25 pg/mL
Range 25 pg/mL-1,600 pg/mL

POLR2A Colorimetric Cell-Based ELISA Kit - EKA50499 from Biomatik

Category ELISA Kit
Prices $399.00
Sizes 96 tests
Sensitivity At least 5000 cells/well
Range Qualitative
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