Rabbit anti-BARD1 Antibody, Affinity Purified

Name	Rabbit anti-BARD1 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0074
Prices	$323.50
Sizes	100 µl
Applications	IP
Species Reactivities	Ape, Marmoset, Chimpanzee, Human, Monkey
Gene	BARD1
Supplier Page	Shop

Product images

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-BARD1 Antibody, Affinity Purified: <B>Cat. No. PLA0074</B>: Detection of Human BARD1 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified Rabbit Anti-BARD1 Antibody <B>PLA0074</B> used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoblotting
Rabbit Anti-BARD1 Antibody, Affinity Purified: Cat. No. PLA0074: Detection of Human BARD1 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified Rabbit Anti-BARD1 Antibody PLA0074 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-BARD1 Antibody, Affinity Purified: <B>Cat. No. PLA0074</B>: Detection of Human BARD1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BARD1 antibody <B>Cat. No. PLA0074</B> used for IP at 6 μg/mg lysate. BARD1 was also immunoprecipitated by BARD1 Antibody B. For blotting immunoprecipitated BARD1, <B>PLA0074</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation
Rabbit Anti-BARD1 Antibody, Affinity Purified: Cat. No. PLA0074: Detection of Human BARD1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BARD1 antibody Cat. No. PLA0074 used for IP at 6 μg/mg lysate. BARD1 was also immunoprecipitated by BARD1 Antibody B. For blotting immunoprecipitated BARD1, PLA0074 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.