Rabbit anti-PTEN Antibody, Affinity Purified

Name	Rabbit anti-PTEN Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0133
Prices	$386.00
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Pig, Monkey, Turkey, Primate, Bovine, Human, Horse, Rabbit, Guinea Pig, Mouse, Chicken, Dog
Gene	PTEN
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-PTEN Antibody, Affinity Purified: <B>Cat. No. PLA0133</B>: Detection of Human PTEN by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-PTEN (<B>Cat. No. PLA0133</B>) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-PTEN Antibody, Affinity Purified: Cat. No. PLA0133: Detection of Human PTEN by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-PTEN (Cat. No. PLA0133) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-PTEN Antibody, Affinity Purified: <B>Cat. No. PLA0133</B>: Detection of Human and Mouse PTEN by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded), 293T (Lane T; 50 μg), and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-PTEN Antibody (<B>Cat. No. PLA0133</B>) used for WB at 0.04 μg (A) and 1 μg/mL (B) and used for immunoprecipitation at 3 μg/mg lysate (B). PTEN was also immunoprecipitated by Rabbit Anti-PTEN Antibodies recognizing different epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Immunoblotting
Rabbit Anti-PTEN Antibody, Affinity Purified: Cat. No. PLA0133: Detection of Human and Mouse PTEN by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded), 293T (Lane T; 50 μg), and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-PTEN Antibody (Cat. No. PLA0133) used for WB at 0.04 μg (A) and 1 μg/mL (B) and used for immunoprecipitation at 3 μg/mg lysate (B). PTEN was also immunoprecipitated by Rabbit Anti-PTEN Antibodies recognizing different epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).