Rabbit anti-PHF8 Antibody, Affinity Purified

Name	Rabbit anti-PHF8 Antibody, Affinity Purified
Supplier	Sigma-Aldrich
Catalog	PLA0211
Prices	$323.50
Sizes	100 µl
Applications	ICC/IF
Species Reactivities	Pig, Ape, Mouse, Dog, Monkey, Rabbit, Human, Horse, Primate, Chimpanzee, Bovine
Gene	PHF8
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Rabbit Anti-PHF8 Antibody, Affinity Purified: <B>Cat. No. PLA0211</B>: Detection of Mouse PHF8 by Immunohistochemistry. Sample: FFPE section of Mouse renal cell carcinoma. Antibody: Affinity purified Rabbit Anti-PHF8 (<B>Cat. No. PLA0211</B>) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-PHF8 Antibody, Affinity Purified: Cat. No. PLA0211: Detection of Mouse PHF8 by Immunohistochemistry. Sample: FFPE section of Mouse renal cell carcinoma. Antibody: Affinity purified Rabbit Anti-PHF8 (Cat. No. PLA0211) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

<B>Immunohistochemistry</B><BR/>Rabbit Anti-PHF8 Antibody, Affinity Purified: <B>Cat. No. PLA0211</B>: Detection of Human PHF8 by Immunohistochemistry. Samples: FFPE serial sections of Human ovarian carcinoma. Antibody: Affinity purified Rabbit Anti-PHF8 (<B>Cat. No. PLA0211</B>) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

Immunohistochemistry
Rabbit Anti-PHF8 Antibody, Affinity Purified: Cat. No. PLA0211: Detection of Human PHF8 by Immunohistochemistry. Samples: FFPE serial sections of Human ovarian carcinoma. Antibody: Affinity purified Rabbit Anti-PHF8 (Cat. No. PLA0211) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Immunofluorescence: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

<B>Immunoblotting</B><BR/>Rabbit Anti-PHF8 Antibody, Affinity Purified: <B>Cat. No. PLA0211</B>: Detection of Human and Mouse PHF8 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, HeLa (RIPA), 293T, Jurkat, and Mouse TCMK-1 cells. Antibodies: Affinity purified Rabbit Anti-PHF8 Antibody <B>PLA0211</B> used for WB at 0.04 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoblotting
Rabbit Anti-PHF8 Antibody, Affinity Purified: Cat. No. PLA0211: Detection of Human and Mouse PHF8 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, HeLa (RIPA), 293T, Jurkat, and Mouse TCMK-1 cells. Antibodies: Affinity purified Rabbit Anti-PHF8 Antibody PLA0211 used for WB at 0.04 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-PHF8 Antibody, Affinity Purified: <B>Cat. No. PLA0211</B>: Detection of Human PHF8 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-PHF8 antibody <B>Cat. No. PLA0211</B> used for IP at 6 μg per reaction. PHF8 was also immunoprecipitated by Rabbit Anti-PHF8 antibody B. For blotting immunoprecipitated PHF8, <B>PLA0211</B> was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunoprecipitation
Rabbit Anti-PHF8 Antibody, Affinity Purified: Cat. No. PLA0211: Detection of Human PHF8 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-PHF8 antibody Cat. No. PLA0211 used for IP at 6 μg per reaction. PHF8 was also immunoprecipitated by Rabbit Anti-PHF8 antibody B. For blotting immunoprecipitated PHF8, PLA0211 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.