Using Western blot, a significant stimulation of JNK 1/2/3 phosphorylation at Thr183/Tyr185 is detected in HEK293 cells treated with anisomycin for 30 minutes (+) compared with untreated cells (-), while no change in total JNK levels is observed.
Using the JNK 1/2/3 assay kits, a significant stimulation of JNK 1/2/3 phosphorylation at Thr183/Tyr185 is detected in HEK293 cells treated with anisomycin for 30 minutes (+) compared with untreated cells (-), while no change in total JNK levels is observed.
A dilution series of recombinant active JNK1alpha was prepared by diluting to various concentrations in Lysis Mix, containing 0.1% BSA. These dilution series were analyzed using the PhosphoTracer phospho-JNK assay. Limit of detection of active rhJNK was around 50 ng/mL.
PhosphoTracer JNK 1/2/3 (pT183/Y185) ELISA Kit (ab119634) used in Sandwich ELISA.