Example standard curve. Serially diluted recombinant mtTFA (ab103784) in the working range of the assay.
Example of serially diluted HeLa in the assay working range.
Demonstration of assay specificity. (A) 25µg/mL HEK 293T and HeLa cell lysates and Human Liver Homogenate (HLH) were assayed by the mtTFA ELISA (ab123455) and mtTFA concentration was interpolated from the standard curve.
Demonstration of assay specificity. Western blots using the mtTFA ELISA detector antibody (ab119684) at 1µg/mL: (B) 20µg of each lysate and (C) 30ng of mtTFA standard protein (ab103784).
Immunocytochemistry image of mtTFA ELISA detector antibody (ab119684) at 1 µg/mL staining Human HDFn cells.