NF kappaB p65 (pS536) SimpleStep ELISA™ Kit
| Name | NF kappaB p65 (pS536) SimpleStep ELISA™ Kit |
|---|---|
| Supplier | Abcam |
| Catalog | ab176647 |
| Category | ELISA Kit |
| Prices | $484.00 |
| Sizes | 1 x 96 tests |
| Assay Type | Semi-quantitative |
| Sample Type | Cell Lysate, Tissue Homogenate |
| Detection | Colorimetric |
| Sensitivity | < 5 µg/ml |
| Range | 6 µg/ml - 600 µg/ml |
| Format | Microplate |
| Applications | ELISA |
| Species Reactivities | Mouse, Human, Rat |
| Gene | RELA |
| Supplier Page | Shop |
Product images
Example of a typical NFĸB p65 (pS536) cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.
Linearity of dilution in representative sample matrices. Cellular lysates were prepared at 3 concentrations in common media containing 1X Cell Extraction Buffer PTR. Data from duplicate measurements of NFĸB p65 (pS536) are normalized and plotted.
Cell line analysis for Total NFĸB p65 from 200 µg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).
Induction of NFĸB p65 (pS536) phosphorylation in MCF-7 cells in response to TNFα treatment. HeLa cells were cultured in 96-well tissue culture plates, and treated (10 min) with a dose-range of TNFα before cell lysis. Data from quadruplicate measurements of NFĸB p65 (pS536) are plotted and compared against total NFĸB p65 protein levels. Comparative NFĸB p65 (pS536) and NFĸB p65 (Total) data also shown by Western Blot.
Product References
Suppression of glia maturation factor expression prevents - Suppression of glia maturation factor expression prevents
Khan MM, Zaheer S, Nehman J, Zaheer A. Neuroscience. 2014 Sep 26;277:196-205.
Glia maturation factor deficiency suppresses 1-methyl-4-phenylpyridinium-induced - Glia maturation factor deficiency suppresses 1-methyl-4-phenylpyridinium-induced
Khan MM, Kempuraj D, Zaheer S, Zaheer A. J Mol Neurosci. 2014 Aug;53(4):590-9.