Example of PDGF-BB standard curve for ab184860
Titration of Human serum with ab184860 within the working range of the assay. Background subtracted data from duplicate measurements are plotted.
Observed PDGF-BB levels in pooled donor normal Human plasma and normal Human platelet-poor plasma (PP-Plasma). Mean Human PDGF-BB values fall within expected normal reference ranges (WHO).
ab184860 was validated for assay specificity against PDGF-A protein. Background-subtracted data values (mean +/- SD) are graphed. In addition, PDGF-A was prepared at 15 ng/mL and assayed for cross reactivity. No cross reactivity or interference was observed.
Observed PDGF-BB levels in individual donor normal Human serum (n=10). Mean Human PDGF-BB is 145 pg/mL and values fall within expected normal reference ranges (WHO).
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 µg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed for endogenous PDGF-BB levels.