Example standard curve of serially diluted purified human PDK4 in the linear working range of the assay.
Example human sample curve of serially diluted human heart homogenate extract in the working range of the assay.
Interference by cell culture media (20F DMEM), Goat serum, and a titration of 1X extraction buffer were evaluated.
PDK4 exists ubiquitous in tissues with highest levels of expression in heart (HHH) and skeletal muscle (HSMH). This ELISA assay applies specifically to human samples. No signal was observed when using rat tissue (rat heart homogenate (RHH)) or mouse tissue (mouse heart homogenate (MHH)).
Assay Specificity was determined by using human PDK1 recombinant protein as negative control.
All lanes : Pyruvate Dehydrogenase Kinase isoform 4 Human ELISA Kit (ab126582)Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900) at 20 µgLane 2 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431) at 20 µgLane 3 : PDK1 protein at 0.1 µgLane 4 : PDK2 protein at 0.1 µgLane 5 : PDK3 protein at 0.1 µgLane 6 : PDK4 protein at 0.1 µg