Example standard curve. Serially diluted recombinant SIRT1 in the working range of the assay.
Example of serially titrated HeLa extract in the assay working range.
The expression level of SIRT1 varies by cell type. Four cell types are analyzed by SIRT1 expression level relative to the Human HepG2 cell line. This relative relationship is confirmed by Western blotting using antibody ab13533.
The SIRT1 detector antibody (ab110304) in this ELISA is available as standalone antibody. In this image Immunocytochemistry of the antibody (ab110304) from this ELISA used to stain Human HDFn cells.