A431 cells were stimulated by different concentrations of EGF for 10 minutes at 37°C.
A431 cells were stimulated by different concentrations of EGF for 30 minutes at 37°C.
Western blot analysis of extracts from 100 ng/ml hEGF treated A431 cells. Phospho-Stat3 (Tyr705) and Anti-Stat3 antibodies were used in both detection assays.
A431 cells were stimulated by different concentrations of EGF for 10 minutes at 37°C.
A431 cells were stimulated by different concentrations of EGF for 30 minutes at 37°C.