TNF alpha Human SimpleStep ELISA® Kit
| Name | TNF alpha Human SimpleStep ELISA® Kit |
|---|---|
| Supplier | Abcam |
| Catalog | ab181421 |
| Category | ELISA Kit |
| Prices | $484.00 |
| Sizes | 1 x 96 tests |
| Assay Type | Sandwich (quantitative) |
| Sample Type | Serum, Plasma, Cell culture media |
| Detection | Colorimetric |
| Sensitivity | 14 pg/ml |
| Range | 31.25 pg/ml - 2000 pg/ml |
| Format | Microplate |
| Applications | ELISA |
| Species Reactivities | Human |
| Gene | TNF |
| Supplier Page | Shop |
Product images
Background-subtracted data values (mean +/- SD) are graphed.
Background-subtracted data values (mean +/- SD) are graphed.
Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2mM L-glutamine, 100 U/mL penicillin and 100 µg/mL streptomycin. Cells were cultured for 2 days at 37°C in the presence or absence of PHA. The concentrations of TNF-alpha were interpolated from the calibration curve and corrected for sample dilution. The mean TNF-alpha concentration was determined to be undetectable in basal media, 1.2 ng/mL in unstimulated PMBC supernatants and 4.7 ng/mL in stimulated PBMC supernatants.