Standard curve of huIL-8 with background signal subtracted (duplicates; +/- SD).
huTNFa measured in biological fluids, background signal subtracted (duplicates +/- SD).
TNFa detected in supernatants from control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), and PMA with the addition of 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. PBMCs were stimulated for 48 hours with 2 % PHA-M (LifeTechnologies). Results shown after background signal was subtracted (duplicates +/- SD).
Representative standard curve using ab100654
Representative standard curve using ab100654