Example ZAP70 standard curve. Background-subtracted data values (mean +/- SD) are graphed. Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Titration of Jurkat extract within the working range of the Zap70 assay. Background subtracted data from duplicate measurements are plotted.
Interpolated values of ZAP70 are graphed for the indicated cells lines based on a extract load of 12.5 µg/mL. The ZAP70 detector antibody was used to analyze the same extracts by western blot (20 µg loaded/lane). The actin blot is included to show the relative loads of each lysate.