ZAP70

Gene Symbol	ZAP70
Entrez Gene	7535
Alt Symbol	SRK, STCD, STD, TZK, ZAP-70
Species	Human
Gene Type	protein-coding
Description	zeta-chain (TCR) associated protein kinase 70kDa
Other Description	70 kDa zeta-associated protein|70 kDa zeta-chain associated protein|syk-related tyrosine kinase|tyrosine-protein kinase ZAP-70|zeta-chain associated protein kinase, 70kD
Swissprots	Q6PIA4 A6NFP4 Q9UBS6 P43403 Q8IXD6
Accessions	AAX93187 EAX01918 EAX01919 P43403 AB083211 BAC43747 AK303341 BAG64404 AK310628 AK310637 AL832287 BC039039 AAH39039 BC053878 AAH53878 L05148 XM_005264015 XP_005264072 XM_006712728 XP_006712791 XM_011511783 XP_011510085 XR_923018 XR_923019 XR_923020 NM_001079 NP_001070 NM_207519 NP_997402
Function	Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, ad
Subcellular Location	Cytoplasm {ECO:0000269|PubMed:9813084}. Cell membrane {ECO:0000269|PubMed:9813084}; Peripheral membrane protein {ECO:0000269|PubMed:9813084}. Note=In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity). {ECO:0000250}.
Tissue Specificity	Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells. {ECO:0000269|PubMed:1423621, ECO:0000269|PubMed:16467082, ECO:0000269|PubMed:9378960}.
Top Pathways	Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, Primary immunodeficiency

• Antibodies
• Assays/Kits
• Proteins/Peptides
• siRNAs/Functional Genomics
• cDNA Clones
• Cell/Tissue/Blood Products

PathScan ® Total Zap-70 Sandwich ELISA Antibody Pair - 7850 from Cell Signaling Technology Category Antibody Pair Prices $459.00 Sizes 1 kit (Reagents for 4 x 96 well plates)

PathScan® Total Zap-70 Sandwich ELISA Kit - 7172 from Cell Signaling Technology Category ELISA Kit Prices $489.00 Sizes 1 kit (96 assays)

PathScan ® Phospho-Zap-70 (Tyr319) Sandwich ELISA Antibody Pair - 7852 from Cell Signaling Technology Category Antibody Pair

PathScan® Phospho-Zap-70 (Tyr319) Sandwich ELISA Kit - 7171 from Cell Signaling Technology Category ELISA Kit

ZAP70 Human SimpleStep ELISA™ Kit - ab173192 from Abcam Category ELISA Kit Prices $484.00 Sizes 1 x 96 tests Sensitivity < 500 pg/ml Range 1.6 ng/ml - 100 ng/ml

Phosphotyrosine ZAP70 / Tyrosine-protein Kinase ZAP-70 ELISA Kit - RAB1010 from Sigma-Aldrich Category ELISA Kit Prices $637.00 Sizes 1 kt

Mouse/Human Phospho-ZAP70 (Tyr315) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1238 from LifeSpan Bioscience Category ELISA Kit Prices $550.00 Sizes 1 plate Range 0.312-20 ng/ml

Mouse/Human/Rat Phospho-ZAP70 (Tyr493) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1650 from LifeSpan Bioscience Category ELISA Kit Prices $550.00 Sizes 1 plate Range 0.312-20 ng/ml

Human ZAP70 ELISA Kit (Sandwich ELISA) - LS-F12991 from LifeSpan Bioscience Category ELISA Kit Prices $600.00 Sizes 1 plate Sensitivity 0.312 Range 0.312-20 ng/ml

Human Total ZAP70 DuoSet IC ELISA - DYC3709-2 from R&D Systems Category ELISA Kit Prices $329.00 Sizes 1 kit (for 2 Plates) Range 625.00 - 40,000 pg/mL

Tyrosine-protein kinase ZAP-70, ZAP70, Human, ELISA Kit - E7846h from ARP American Research Products Category ELISA Kit Prices $736.00 Sizes 96 t Range 0.312-20 ng/mL

zeta-chain (TCR) associated protein kinase 70kDa, ELISA Kit - MBS9338098 from MyBioSource Category ELISA Kit Prices $435.00, $600.00, $2,650.00, $5,205.00 Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

ZAP-70 (Phospho-Tyr292) Colorimetric Cell-Based ELISA Kit - CBP1234 from Assay Biotechnology Category ELISA Kit Prices $480.00, $960.00, $1,440.00 Sizes 1 kit, 2 kits, 3 kits Range > 5000 Cells

ZAP-70 (Phospho-Tyr315) Colorimetric Cell-Based ELISA Kit - CBP1235 from Assay Biotechnology Category ELISA Kit Prices $480.00, $960.00, $1,440.00 Sizes 1 kit, 2 kits, 3 kits Range > 5000 Cells

ZAP-70 (Phospho-Tyr493) Colorimetric Cell-Based ELISA Kit - CBP1709 from Assay Biotechnology Category ELISA Kit Prices $480.00, $960.00, $1,440.00 Sizes 1 kit, 2 kits, 3 kits Range > 5000 Cells

Mouse ZAP70 ELISA Kit (Custom ELISA) - LS-F20137 from LifeSpan Bioscience Category ELISA Kit Prices $600.00 Sizes 1 plate Sensitivity To be determined Range 0.312-20 ng/ml

Human Phospho-ZAP70 (Y493) ELISA - PEL-ZAP70-Y493 from RayBiotech Category ELISA Kit Prices $481.00, $866.00, $1,924.00 Sizes 1 plate kit, 2 plate kit, 5 plate kit

Phospho-ZAP70 Antibody Duo (pY319, pY493) - ARG30043 from Arigo Biolaboratories Category Antibody Pair

Lck / Phospho-ZAP70 Antibody Duo (Lck, pZAP70 Y319) - ARG30096 from Arigo Biolaboratories Category Antibody Pair

Phospho-ZAP70 Antibody Duo (Total, pY319) - ARG30033 from Arigo Biolaboratories Category Antibody Pair

ZAP-70 (Phospho-Tyr292) ELISA Kit - DEIA-XYA2024 from Creative Diagnostics Category ELISA Kit

ZAP-70 (Phospho-Tyr315) ELISA Kit - DEIA-XYA2025 from Creative Diagnostics Category ELISA Kit

ZAP-70 (Phospho-Tyr493) ELISA Kit - DEIA-XYA2026 from Creative Diagnostics Category ELISA Kit

ZAP70 - EKH4750 from Nordic BioSite Sensitivity 0.188ng/ml Range 0.312-20ng/ml

ZAP70 ELISA Kit (Human) : 96 Wells (OKEH01831) - OKEH01831 from Aviva Systems Biology Category ELISA Kit Prices $480.00 Sizes 96 wells Sensitivity < 0.2 ng/mL Range 0.312 - 20 ng/mL

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