PathScan ® Total Src Sandwich ELISA Kit
| Name | PathScan ® Total Src Sandwich ELISA Kit |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 7984 |
| Category | ELISA Kit |
| Prices | $489.00 |
| Sizes | 1 kit (96 assays) |
| Assay Type | Sandwich |
| Applications | ELISA |
| Description | The PathScan ® Total Src Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total Src protein |
| Gene | SRC |
| Supplier Page | Shop |
Product images
Figure 1. Constitutive phosphorylation of Src at Tyr416 in Calu-3 cells as detected by the PathScan® Phospho-Src (Tyr416) Sandwich ELISA Kit #7953. Treatment of lysate with λ-phosphatase significantly reduces Src phosphorylation but does not alter the levels of total Src protein as detected by the PathScan® Total Src Sandwich ELISA kit #7984. Absorbance at 450 nm is shown in the top figure while the corresponding western blots using Src (L4A1) Mouse mAb #2110 (left) or Phospho-Src Family (Tyr416) (100F9) Rabbit mAb #2113 (right) are shown in the bottom figure.
Figure 2. The relationship between lysate protein concentration from untreated and λ-phosphatase treated lysate from Calu-3 cells and the absorbance at 450 nm is shown.